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Mutational hotspots in ι-crystallin. (A) Ribbon model of the ι-crystallin β-barrel, with positions of amino acid replacements (compared with rat CRBP I) in red and with 3,4-didehydroretinol in green stick representation. Orientation as in Fig. 3 Upper. (B and C) Comparison of the retinoid binding pockets of ι-crystallin and rat CRBP I, respectively. Amino acid side chains are drawn as sticks, colored by atom type. The retinoid is shown in purple sticks.
