Figure 4.

ChtA drives wild-type cells into the prestalk region of the sorocarp: A shows the results of mixing 20% wild-type cells marked with β-gal driven by the ecmAO prestalk specific promoter and 80% unlabeled wild-type cells (AX3). In B, 20% AX3 with an ecmAO driven β-gal was mixed with 80% unlabeled chtA cells. C, E, and G show a mixture of 20% wild type labeled with the prespore psA promoter driving β-gal and 80% unlabeled wild type (AX3). D, F, and H show what happens when the unlabeled wild-type cells are replaced with the chtA mutant.