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. 2003 Jun 15;31(12):3174–3184. doi: 10.1093/nar/gkg429

Figure 1.

Figure 1

Translation of Cx41 is controlled by three uORFs. (A) Sequence of the X.laevis Cx41 5′ UTR. (GenBank™ accession no. AF238222). uAUG codons are underlined, termination codons of the uORFs are in italics, and the GFP AUG is in bold (nucleotides 171–173). (B) Schematic representation of the three overlapping uORFs in the Cx41 5′ UTR. uORF2 and uORF3 are in the same reading frame and share a termination codon, they are also in the same reading frame as the main ORF. uORF1 is in a different reading frame. (C) Schematic representations of the ORFs present in the different mutants used in this study. For a detailed description of these and other mutants, see Table 1. The relative translation efficiencies of the different mutants after injection into one cell-stage Xenopus embryos are based on the results presented in Figure 1. The #23 mutant was not injected and was only used for in vitro assays. N.D.: not done.