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In vitro translation of codon bias transcripts. The indicated Cx41–GFP transcripts were translated in a rabbit reticulocyte lysate in the presence of [35S]methionine. The resulting proteins were separated on a 15% 30:0.18 acrylamide/bisacrylamide gel. Deletion of uAUG2 and uAUG3 slightly changed the migration of #1 GFP due to two amino acid changes.
