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Wild-type PPR1 mRNA accumulates in hrp1-3 yeast cells at the non-permissive temperature. PPR1 mRNA accumulation in Y137 (wild-type) and Y191 (hrp1-3) at room temperature (rt) and after 1 h at 37°C. Y137 and Y191 were grown in YAPD. Northern blots were prepared using 15 µg of total RNA and hybridized with radiolabeled PPR1, PGK1 and ScR1 DNA. The relative PPR1 steady-state levels are indicated under the northern blot hybridized with radiolabeled PPR1 DNA.
