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. 2003 Jun 15;31(12):3157–3165. doi: 10.1093/nar/gkg430

Figure 5.

Figure 5

mRNAs encoded by the PPR1-ACT1 and the mini-PPR1 gene constructs are degraded more rapidly in AAY334, a UPF1 yeast strain, than the isogenic upf1Δ yeast strain, AAY335. mRNA half-lives for PPR1-ACT1 (A) and PPR1 mini-mRNA (B) were determined from northern blots of total RNA harvested from UPF1 and upf1Δ yeast cells grown in CM-leucine at time points following the arrest of transcription as described in the legend of Figure 1B. The time points at which the cells were harvested after inhibition of transcription are shown above the PhosphorImages and are different for the mRNAs encoded by the PPR1-ACT1 construct and the mini-PPR1 gene construct. The upper band in the PhosphorImages of (A) is the endogenous PPR1 mRNA.