(A) Time course studies of glycosylase activities of the wild-type and mutant MutY proteins with A/GO-containing DNA at 20°C. Reactions were similar to Figure 4B with 0.45 nM proteins but were performed at 20°C. Wild-type MutY (WT, solid circle), R249A-MutY (solid triangle) and F294A-MutY (open circle). (B) The temperature effect on glycosylase activities of the wild-type MutY and F294A-MutY with A/GO-containing DNA. Reactions were performed at 4, 10, 15, 20 and 30°C for 30 min with 0.45 nM proteins.