Figure 2.

Abnormal T lymphocyte development in Atm^y/y mice. (A) Average pairwise comparisons of weight, total numbers of thymocytes, and splenocytes from 10 same-sex littermates pairs 1–5 mo old. (B) (Upper) The average reduction of the total number of each Atm^y/y thymocyte subpopulations (DN, CD4⁻8⁻; DP; CD4⁺8⁺; CD4⁺SP, CD4⁺8⁻; CD8⁺SP, CD4⁻8⁺) from seven littermate pairs. (Lower) FACS analysis of T cell development in Atm^y/y thymuses. (C) Histogram of CD3 surface expression in Atm^y/y thymocytes (y/y). (Lower) FSC (forward site scatter) using a lymphocyte gate on CD3^low and CD3^int DP cells, respectively. (D) Splenic T cells in Atm^y/y mice (y/y) were reduced in numbers, but their surface phenotype (TCRβ/CD3 expression) appeared to be normal. Numbers indicate the percentage of splenic T cells (CD3⁺) and B cells (B220⁺).