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. 2000 Mar 21;97(7):3358–3363. doi: 10.1073/pnas.050582797

Figure 1.

Figure 1

Gene targeting at the B18/4 locus. Schematic physical maps of the B18/4 target locus, the G125 repair construct, and the recombination product obtained after precise gene replacement are shown. Relevant genes, promoters, and polyadenylation sites are shown as large arrows (MTX, methotrexate resistance gene; pBR322, part of pBR322, including the origin of replication and the Ampicillin resistance gene; ocs, octopine synthase polyadenylation signal; P35S, CaMV 35S promoter; nptII ΔC, carboxyl-terminal deletion mutant of nptII), the T-DNA left (Bl) and right (Br) borders as small arrows, and characterized flanking tobacco DNA sequences as small boxes. The map is not drawn to scale. The size of fragments obtained after restriction of B18/4 target locus DNA with EcoRI, HindIII, ScaI, and SpeI is indicated. The primers used in the PCR analysis of recombination events at the B18/4 locus are shown schematically (1, hmw2404; 2, b18rbin; 3, 5634; 4, 2391; 5, hmw35S).