Figure 2.

Ecc-15 persists in developing larvae and pupae. Bacterial persistence was measured in wild-type (Or^R) and imd/imd mutant lines by plating appropriate dilutions of homogenates of five surface-sterilized larvae or pupae that were naturally infected with rifampicin-resistant strains of Ecc-15 (Ecc-15 rif^R) and Ecc-2046 (Ecc-2046 rif^R) and collected at different times after infection. Bacterial counts were obtained by plating the larval and pupal homogenates on LB medium containing rifampicin (100 μg/ml; ref. 14). The number of colony-forming units (cfu) per larva obtained at each time point after infection represents the mean of three independent measurements. L3, third instar larvae; P, pupae.