Figure 3.

Fate of Ecc-15 bacteria in the larvae. The localization of Ecc-15 within larvae was determined by using a strain of Ecc-15 (Ecc-15-GFP) that expresses the GFP reporter gene. (A) In a larva naturally infected by Ecc-15-GFP, bacteria are clearly present in the pharynx and anterior midgut. (B) In a larva infected by Ecc-15-GFP, bacteria are present in the anterior spiracles. (C) Hemolymph collected from larvae infected for 16 h with Ecc-15 shows GFP-marked bacteria phagocytosed by hemocytes. P, pharynx; AM, anterior midgut.