Table 2.
Antigen specificity, V-gene usage, and location of codon insertions and/or deletions in individual antigen-specific Fabs
| Number of Codons | ||||||
| Specificitya | Donorsb | Clone | Isotype | V genec | Ins/Del | Locationd |
| PA | 1/1 | 001.46F1 | κ/G1 | A27 | 1 del | CDR1 |
| PPS 6B | 3/6 | 003.6F2 | κ/G2 | VH3-15 | 2 ins | CDR2 |
| 023.16B11 | λ /A2 | VH3-23 | 4 del | CDR3 | ||
| 010.3H11 | κ/A2 | B3 | 6 del | CDR1 | ||
| 023.20H2 | λ/A2 | V1-4 | 1 ins | FR2 | ||
| PPS 14 | 2/3 | 023.14H10 | λ/G2 | VH1-46 | 1 del | CDR2 |
| 023.9E5 | λ/G2 | VH1-46 | 2 del | CDR2 | ||
| 011.11A11 | λ/G2 | VH3-7 | 1 ins | FR1 | ||
| 011.5G1 | κ/A2 | VH3-23 | 1 del | CDR2 | ||
| PPS 23F | 3/7 | 018.6G5 | κ/G2 | VH3-7 | 2 ins | FRI |
| 027.064 | κ/G2 | VH3-23 | 1 ins | CDR2 | ||
| 008.4B7 | κ/G2 | VH4-4 | 2 ins | CDR1 | ||
aAntigen binding specificity of human antibody Fabs. bNumber of donors from which Fabs containing insertions or deletions were isolated over the number of donors analyzed. cGermline gene modified in the Fab. Kappa L chain nomenclature as described in [20], lambda L chain nomenclature as described in [21], H chain nomenclature as described in [23]. dLocation of the modification; CDR and frameworks regions are as described in [24].