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Nanoelectropulse-induced phospholipid rearrangement and PS externalization does not require calcium. FM1-43 fluorescence intensification was photometrically measured 5 min after exposure to 20, 3 ns, 6.0 MV/m pulses. Experimental samples had either 5 mM EGTA in the medium (added immediately before pulse delivery) or were loaded with 10 μM BAPTA-AM for 60 min before pulsing.
