Culicoides sonorensis Con A binding protein and SGE. (A), The equivalent of 10 pairs of salivary glands (2 μg) was loaded on a reducing SDS-PAGE gel, and the proteins were stained with silver stain. (B), Con A–binding protein of 66 kDa, eluted from a lectin affinity chromatography column, reacts with biotinylated Con A on a lectin blot. An identical affinity chromatography column was processed in parallel with only PBS in place of SGE as a negative control (data not shown). (B) is representative of two independent experiments.