Figure 1.

The human CCR5-specific CCR5–02 mAb interferes neither with RANTES binding nor function. (A) CCR5-transfected HEK-293 cells were incubated alone or with 10 nM RANTES (30 min, 4°C). After washing, cells were incubated with biotin-labeled CCR5–02 mAbs, as described. An isotype-matched mAb was used as control. (B) RANTES-induced Ca²⁺ mobilization in CCR5-transfected HEK-293 cells was unaffected by pretreatment with 10 μg/ml CCR5–02 mAb or isotype-matched control mAb (30 min, 37°C). As a control, Ca²⁺ mobilization by CCR5–02 mAb (10 μg/ml) is shown. (C) RANTES-induced CCR5-transfected HEK-293 cell migration was unaffected by pretreatment with CCR5–02 mAbs (10 μg/ml, 30 min 37°C) or isotype-matched control mAbs. The figure depicts one of five experiments performed, with SD indicated.