Figure 4.

The CCR5–02 mAb triggers receptor dimerization. CCR5-transfected HEK-293 cells were stimulated with 10 nM RANTES, 10 μg/ml CCR5–02 mAb, or 10 μg/ml isotype-matched control mAb (60 sec, 37°C), and crosslinked by using 1 mM disuccinimidyl suberate. Cell lysates were immunoprecipitated with CCR5–03 mAb, electrophoresed, and transferred to nitrocellulose membranes. The Western blot was analyzed with CCR5–01 mAb; as a positive control, CCR5-transfected HEK-293 cell lysates were immunoblotted with CCR5–01 mAb. Arrows indicate the monomer and the dimer.