FIGURE 3.

Identification of RNA species co-immunoprecipitated by ProtA–Slx9p. Total cell extracts from exponentially growing strains slx9::KAN (Y14711), slx9::KAN+pURA3–ProtA::slx9, and SC1413 (TAP::Rio2) were prepared and treated with IgG beads. (A,B) Northern analysis of co-precipitated RNA (IP) or nonbound RNA (SUP) on agarose gels using probe 1 and 2 (see Fig. 1A). (C) Northern analysis of co-precipitated RNA separated on a 8% polyacrylamide gel using a probe complementary to the C′ box of U3 snoRNA.