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Reverse transcription analysis of pre-rRNA processing intermediates. RNA was isolated from strains Y10000 (EUROSCARF wild-type, lane 1), slx9::KAN (Y14711) (lane 2), slx9::LEU2 (YCV711) (lane 3), and slx9::LEU2+pHIS–SLX9 (lane 4) and used as a template for reverse transcription using probe 3 (see Fig. 1A). The bands indicated by the asterisks represent artificial stops.
