FIGURE 6.

Effect of the deletion of SLX9 in cells expressing the mutant genes rrp5-Δ3 and rrp5-Δ6. (A) Growth. Cells of strain YJV306 (rrp5-Δ3), YJV207 (rrp5-Δ6), YJV163 (wild-type RRP5), rrp5-Δ3Δslx9 (YRB154Δ3), rrp5-Δ6Δslx9 (YRB154Δ6), and RRP5Δslx9 (YRB154), were streaked out on YPGAL and YPD plates and grown at 30°C for 3–4 d. (B–D) Effect on pre-rRNA processing. The strains were grown on YPGAL to mid-exponential phase and then shifted to YPD medium for 24 h. Total RNA was isolated from equal amounts of cells, separated on 1.2% agarose gels, and subjected to ethidium bromide staining and Northern analysis. (B) Northern analysis to visualize mature 18S and 25S rRNA. The 18S:25S ratio was established by Northern analysis using probes that detect 18S and 25S rRNA. The signals obtained by phosphor imaging were quantitated using the ImageQuant software tools. (C) Northern analysis using probe 1. (D) Northern analysis using probe 2. See Figure 1A for the positions of the probes.