Figure 2.

PKCθ selectively activates NF-κB and the CD28RE/AP-1 element of the IL-2 promoter in a T cell-specific manner. The 1 × 10⁷ Jurkat T cells (a and b) or 2 × 10⁶ 293T cells (c) were transfected with CD28RE/AP-1 (a) or NF-κB-Luc (b and c) reporters (5 μg each) in the presence of empty vector (pEF; −) or constitutively active (A/E) PKC-θ, -α, -ζ or -ɛ mutants (10 μg each). After 24 h, cells were lysed and luciferase activity was quantified. The expression level of the different PKCs was analyzed by Western blotting with an anti-Xpress antibody, and anti-actin immunoblotting was used as a control for protein loading (Lower).