Figure 7.

Tethering and rolling of NK cells on P- and E- selectin under flow conditions. NK cells were incubated with the indicated mAb or with mocarhagin, as described in Fig. 2A; then, NK cells were diluted and perfused at 1.5 dyne/cm² through the chamber. Interactions of NK cells with P- (solid bars) or E-selectin (shaded bars) were abolished in the presence of 5 mM EDTA in the perfusing medium (data not shown) or when the substrates were preincubated for 10 min with 10 μg/ml of anti-P-selectin or anti- E-selectin (data not shown). No inhibition of rolling on E-selectin was observed when NK cells were incubated with mocarhagin. The accumulated numbers of rolling cells were determined by analysis of videotapes in which at least six fields randomly chosen were counted throughout the last 2 min of the flow period. The percentage of rolling NK cells for each condition is calculated relative to untreated controls. Data represent mean ± SEM of results from four experiments. *, P < 0.05.