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. 2000 Mar 21;97(7):3406–3411. doi: 10.1073/pnas.060026497

Figure 1.

Figure 1

Induction of long-term CD8+ T cell survival by short-term IL-4 exposure during TCR stimulation. Donor/total CD8+ T cell ratios were determined as a function of time for individual B10.TL hosts (400R-irradiated) that had received indicated numbers of 2C CD8+ T cells activated in culture by B10.A peritoneal cells without IL-4 addition (a) or 2C CD8+ T cells activated in culture by B10.A peritoneal cells + IL-4 (b). Each line trace represents data derived from a single B10.TL host over time. (c) Criteria for naive CD8+ T cells. Correlated CD44/CD62L expression is shown for 2C tg CD8+4− T cells, with a box marking naive cells. CD8+CD4−CD44lowCD62Lhi was the criteria for sorting naive CD8+ T cells. (d) PBLs from a B10.TL mouse that received 8 × 106 activated 2C CD8+ T cells 109 days previously were stained with F-anti-Thy-1.2 (30H12) + Cy5-anti-CD8 (53–6.7); regions of donor and host CD8+ cells are marked. (e) 2C naive CD8+ T cells (4 × 106) activated by B10.A B cell blasts + IL-4 were transferred into a B10.TL host. Day 168 posttransfer, host spleen cells were stained with F-anti-CD8.2 + Cy5-anti-CD44 + TR-anti-CD62L; CD44/CD62L-correlated expression was restricted to CD8.2+ donor cells. (f) 2C naive CD8+ T cells (10 × 106) activated by B10.A peritoneal cells + IL-4 were transferred into a B10.TL host. Day 98 posttransfer, donor cell CD44/CD62L-correlated expression was performed as in e.