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Southern blot analysis of BamHI/EcoRI-digested tail DNA from 4E3 (A) and 5B6 (B) transgenic offspring. The TCR αβ transgenes of transgenic (TG) offspring from lines 1736, 1740 (4E3), and 1858 (5B6) are indicated by arrows. Nontransgenic littermates (NLM) are shown as controls. Southern blot analysis was carried out as described by using 32P-labeled 4E3 and 5B6 transgene-specific VJα and VDJβ probes.
