Figure 4.

Responses of transgenic T cells to PLP-139–151. (A) Proliferative response of T cells from 4E3- and 5B6-transgenic mice to PLP-139–151. Splenocytes from unimmunized 4E3 (line 1740) and 5B6 TCR-transgenic (line 1858) and nontransgenic littermate (NLM) mice were cultured with indicated concentrations of PLP-139–151 for 4 days. Proliferative responses were determined by [³H]thymidine incorporation analysis. (B) Transgenic T cells respond to PLP-139–151 by IL-2 and IFN-γ production. Cultures of splenocytes from 4E3 (line 1740) and 5B6 TCR-transgenic (line 1858) and nontransgenic littermate (NLM) mice were stimulated with PLP-139–151 for 40 h. Culture supernatants were assayed in duplicates for cytokine production by ELISA. Data from one of three experiments are shown.