The number of β-galactosidase-positive cells after intracerebral injection. Animals were injected with rAAV2βgal, rAAV4βgal, or rAAV5βgal into the ventricle or striatum of mice, and brains taken at the times indicated. Blocks (2.6 mm, surrounding the injection site) were sectioned, processed for β-galactosidase histochemistry, and transgene-positive cells were counted. Data represent mean ± SEM. *, P < 0.05; **, P < 0.005.