Figure 2.

Effect of dietary cholesterol on gallbladder bile concentrations (A) and percent saturation (B) of cholesterol in WT and apoE knockout mice: WT (E+/+) and apoE knockout (E−/−) mice were fed for 3 wk with 0.02% (open bars) and 0.5% cholesterol (filled bars). After 3 wk, the animals were fasted, the peritoneal cavity was exposed, and gallbladder bile was aspirated. Biliary cholesterol concentrations of cholesterol were measured and percent saturation was calculated as described in Experimental Procedures. Data displayed are the mean ± SD of five animals in each group.