Figure 1.

Generation of transgenic mice. (a) Schematic drawing of the K5 GLI-1 transgene. The transgene contains the bovine K5 promoter, the rabbit β-globin intron, the human GLI-1 cDNA, and the SV40 poly(A) signal. (b) GLI-1 transgenic founder (founder 2) showing a tumor ulceration and the nodule (arrow) on the back. (c) RT-PCR analysis of K5 GLI-1 and β-actin mRNA in dorsal skin of the transgenic founders 1 to 3 (1–3). Sizes of the specific PCR products are 553 bp for β-actin and 370 bp for K5 GLI-1. Lane M pBR322-MspI ladder (New England Biolabs).