Expression of GLI-1 target genes in K5 GLI-1 transgenic skin and tumors (K5 GLI-1) (a, c, e, and g) and in wild-type skin (WT) (b, d, and f). High expression of Gli-1 is seen in a transgenic BCC and adjacent epidermis (a), but no signal was detected in wild-type skin (b). Ptch1 (c and d) and Bcl-2 (e and f) are both overexpressed in transgenic epidermis and tumor cells compared with wild-type skin. However, single cells in the lower parts of the hair follicle in wild-type skin show positive immunostaining for Ptch1 (d). (g) Sections of a BCC showing scattered tumor cells with immunoreactivity for p53 (brown precipitate). Bar = 92 μm (a–f), 54 μm (g).