Figure 6.

Amplification of neu in mammary tumors. Southern analysis showing the amplification of the recombinant allele (4.5 kb) in respect to the wild-type allele (7.5 kb) in the MMTV-Cre Flneo NeuNT tumors (TM1–6). The TM1 adjacent gland (TM1 Adj. Gl.) also is shown, illustrating that detectable amplification has not occurred in the normal mammary gland. The samples were restricted with HindIII and probed with the external probe using the same strategy described in Fig. 1. Interestingly, a new band has appeared above the wild-type band in TM2, TM3, and TM5, likely a result of the amplification process. This Southern analysis was subjected to a quantitative PhosphorImager analysis where the increase in neuNT copy number was determined relative to the wild-type allele. The results are shown in Table 1.