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. 2006 Oct 13;7:259. doi: 10.1186/1471-2164-7-259

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Copyright © 2006 Fico and Mahillon; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Organization of toxin and antitoxin genes frompGI1 of B. thuringiensis H1.1. A) pGI1 contains a mobilization gene (mob1), a replication gene (rep1), a hypothetical transcriptional regulator (ORF5), three small cryptic ORFs (URF94, URF71, URF88) and the putative toxin-antitoxin system tasB and tasA. TasB displays similarities with the Doc toxin of bacteriophage P1. The upstream ORF, tasA, could code for the antitoxin counterpart. Putative double- and single-strand origins are also indicated. B) To assess the function of tasA and tasB, different parts of this putative toxin-antitoxin system have been amplified for cloning purposes. This figure is a scaled representation of the primers and amplimers used in cloning experiments (See Table 2 for details). Amplimer names are shown in the left column.