Skip to main content
. 2006 Nov 1;116(11):3060–3069. doi: 10.1172/JCI27341

Figure 5. Inhibition of neuronal TGF-β signaling increases Aβ, shAPP and CTF in neuroblastoma cells.

Figure 5

(A and B) B103-APPwt cells were transiently transfected with TβRIIΔk and GFP and sorted via fluorescence-activated cell sorting (A) or treated with 1 μM SB505124 to inhibit ALK5 signaling (B). Total secreted Aβ was measured via ELISA 5 days after treatment. (C) Representative Western blots of cell pellets (hAPP, CTFα, CTFβ) or supernatants (shAPPα, shAPPβ) of B103-APPwt cells or B103 cells lacking APP treated with 0 or 1 μM SB505124 and probed with antibodies against total hAPP, CTFα, CTFβ, shAPPα, and shAPPβ. (D–F) Signal intensities of total hAPP (D), shAPPα and shAPPβ (E), and CTFα and CTFβ (F) were quantified and normalized against hAPP levels (E and F), and the ratio between shAPPα and shAPPβ was calculated (E). Data are mean ± SEM of triplicate wells of 1 representative experiment. **P < 0.01, ***P < 0.001; Student’s t test.