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. 2006 Nov 1;116(11):2945–2954. doi: 10.1172/JCI28721

Figure 7. Combined IGF-1 supplementation and IKK2 inhibition enhances protection against muscle atrophy.

Figure 7

(A) Comparison of gastrocnemius weights from control (black line), Ikk2mko (dashed line), mIgf-1 (light blue line), and mIgf-1/Ikk2f/f (dark blue line) mice at 7, 14, and 28 days after denervation. Values represent denervated muscle weights after normalization to sham-operated muscle weight (n = 5 mice per time point). (B–I) Enhanced maintenance of muscle-fiber size in gastrocnemius muscle from mIgf-1 and mIgf-1/Ikk2f/f denervated muscle. Magnification, ×100 (B–E); ×200 (F–I). (J) Sustenance in the gastrocnemius fiber CSA in Ikk2mko, mIgf-1, and mIgf-1/Ikk2f/f atrophied muscle. (K) MuRF1 and (L) atrogin-1/MAFbx mRNA levels in gastrocnemius muscles of control (n = 4), Ikk2mko (n = 4), mIgf-1 (n = 3), and mIgf-1/Ikk2f/f (n = 4) muscles as determined by quantitative real-time RT-PCR. Denervated values were normalized to nondenervated values. **Mean values differ significantly from those of control (P < 0.002).