Figure 2.

Patterns of cre-loxP recombination and Western blot analysis of Tfam, ATP8, and actin protein levels. (A) Southern blot analysis of Tfam genotypes in different tissues of E18.5 knockouts (Tfam^loxP/Tfam^loxP, +/Myhca-cre) obtained by the standard mating. The knockout allele (Tfam⁻) is present only in the heart of the knockout embryos. (B) Western blot analyses of Tfam and actin protein levels in different organs of a standard-mating E18.5 knockout (Tfam^loxP/Tfam^loxP, +/Myhca-cre; L/L, cre) and a littermate control (Tfam^loxP/Tfam^loxP; L/L). (C) Western blot analyses of Tfam, ATP8, and actin protein levels in hearts of standard- or mutant-mating E18.5 knockouts (Tfam^loxP/Tfam^loxP, +/Myhca-cre; L/L, cre) and littermate controls (Tfam^loxP/Tfam^loxP; L/L). (D) Western blot analyses of Tfam and actin protein levels in different organs of a standard-mating adult knockout (Tfam^loxP/Tfam^loxP, +/Myhca-cre; L/L, cre) and a littermate control (Tfam^loxP/Tfam^loxP; L/L).