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. 2005 Nov 7;171(3):559–568. doi: 10.1083/jcb.200504015

Figure 2.

Figure 2.

Apoptotic activities of CCN proteins in Rat1a fibroblasts. (A) Cells were grown in 6-well plates and treated with the indicated concentrations of soluble CCN1 for 24 h, followed by fixation and scoring for apoptosis. (B) Cells were pretreated for 1 h with 25 μM cycloheximide and 40 μM DRB before further incubation for 6 h with or without 10 μg/ml CCN1. Cells were fixed and scored for apoptosis. (C) Cells were grown in tissue culture dishes in 10% serum, washed, and maintained in medium with 0% FBS, 2% FBS, 100 ng/ml EGF, or 10 ng/ml of basic FGF, in the presence or absence of 10 mg/ml CCN1 for 24 h before scoring for apoptosis. (D) Cells were adhered to tissue culture dishes or dishes coated with CCN1, CCN2, or PLL (10 mg/ml each) and maintained in medium containing 0.5% FBS with or without soluble CCN1 or CCN2 for 24 h before apoptosis assay. Error bars represent ± SD from experiments done in triplicate.