CoCoA and β-catenin synergistically enhance transcriptional activation by AR and LEF1. (A) CV-1 cells were transfected in 12-well plates with MMTV-LUC reporter plasmid (200 ng), pSV40-AR (50 ng), pSG5.HA-CoCoA (200 ng), and pSG5.HA-β-catenin (200 ng) as indicated, and grown in medium containing 20 nM DHT. Cell extracts were assayed for luciferase activity. (B) CV-1 cells were transfected with pGL3OT reporter plasmid (200 ng), pSG5.HA-LEF1 (10 ng), pSG5.HA-β-catenin (200 ng), and pSG5.HA-CoCoA (200 or 400 ng) as indicated. Results shown are from a single experiment and representative of three independent experiments.