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. Author manuscript; available in PMC: 2006 Dec 15.
Published in final edited form as: J Immunol. 2005 Dec 15;175(12):7829–7836. doi: 10.4049/jimmunol.175.12.7829

FIGURE 6. CD80 cytoplasmic domain regulated both CD28 and CTLA-4 segregation from the TCR.

FIGURE 6

Both naïve and previously activated primary 5C.C7 T cells were stimulated CD80FLYFP or CD80TL-YFP CHO pulsed with 100 nM MCC88-103 peptide. Cells were fixed after T-CHO cells had been interacting for 30 min at 37°C. At this time point, CTLA-4 was not detected on naïve T cells. The color overlay images show CTLA-4 in red, CD28 in red and CD80 in green. The white dashed line represents the outer boundary of the T cell. Images are cross-section of a 3-D plane rotated to en face view. Scale bar = 2 μm. The localization of CD80FL (A) and CD80TL (B) modulated the localization of CD28 respect to CD80 in the IS of naive T cells. The localization of CD80FL-YFP (C) and CD80TL-YFP (D) modulated the localization of CD28 and CTLA-4 respect to CD80 in the IS of previously activated primary T cells. d6-d8 5C.C7 T cells were used.

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