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. Author manuscript; available in PMC: 2006 Oct 30.
Published in final edited form as: J Clin Endocrinol Metab. 2004 Dec 21;90(3):1607–1612. doi: 10.1210/jc.2004-0868

Fig. 4.

Fig. 4

W147A inhibits prolactin and IGFBP-1 production by decidualizing human endometrial stromal cells. Levels of prolactin (A) and IGFBP-1 (B) in supernatants from confluent stromal cells (n = 3) treated for 16 d without (□) or with (■) 0.5 mm 8-Br-c AMP, or 8-Br-cAMP with increasing concentrations of W147A ([unk], 250 ng/ml; [unk], 1 μg/ml). Data are expressed as the mean ± sem. C, Inhibition of cAMP-induced secretion of prolactin (■) and IGFBP-1 ([unk]) in the presence of 1 μg/ml W147A, expressed as a percentage of the cAMP-treated control. Prolactin and IGFBP-1 levels are significantly lower in the presence of W147A (P < 0.05 on d 3–16). D, Cell viability in the presence of 8-Br-cAMP and W147A assessed using the WST-1 colorimetric assay and expressed as the mean OD450nm ± sem. PRL, Prolactin.