Figure 1.

Hybrid peptide binding to hsp70. The ability of OVA-BiP hybrid peptide to bind hsp70 was measured by incubating 72 μM ¹⁴C-labeled OVA-BiP with 50 μg hsp70 at 37°C for 30 min in the presence or absence of 2 mM ADP, 2 mM ATP, or 416 μM OVA. The samples were loaded onto a Sephacryl S-300 column, and fractions were counted in scintillation liquid. The binding assays were performed in the absence (○) or presence of ADP (●), ATP (♦), or unlabeled OVA peptide (□).