Figure 1.

Yeast Two-Hybrid Assay of Interactions between Three Pi SKs and Three F-Box Proteins.

Pi FBP2011 and Pi FBP23 are F-box proteins that are likely encoded by genes unlinked to the S-locus. The bait vector is pGBD-C1, and the prey vector is pGAD-C1; p53 is a tumor suppressor and was used here as a negative control. Six colonies of yeast SFY526 coexpressing a pair of bait and prey proteins were streaked on filter paper for β-galactosidase activity assay. The paper was incubated in an X-Gal–containing solution (Breeden and Nasmyth, 1985) for 1 h at 30°C.