Figure 2.

FAMA Expression Pattern and Loss-of-Function Phenotypes.

(A) RT-PCR of FAMA from fama-1 allele and Col control.

(B) and (C) Differential interference contrast (DIC) images of wild-type and fama-1 cotyledon epidermis 7 d after germination (DAG).

(D) Two-month-old fama-1 plant. Bar = 1 cm.

(E) and (F) Expression of PRO_FAMA:GFP and PRO_FAMA:GFP-FAMA, respectively, in Col cotyledon epidermis. Arrow in (F) points to earliest expression in GMCs.

(G) to (P) Expression of cell fate and cell division markers in fama-1. Bar = 10 μm.

(G) PRO_TMM:TMM-GFP.

(H) and (I) CYCB1;2:GUS in Col and fama-1, respectively.

(J) to (L) CDKB1;1:GUS in Col at 5 DAG (J) and 10-DAG (K) and fama-1 at 10 DAG (L). Note the prolonged expression of this marker in fama-1.

(M) and (N) Mature stomata marker E1728:GFP in Col and fama-1, respectively.

(O) PRO_FLP:GUS/GFP in fama-1.

(P) PRO_FAMA:GFP in fama-1.