Figure 3.

FAMA Gain-of-Function Phenotypes.

(A) to (F) The 7-DAG PRO_EST:FAMA seedling germinated on estrogen.

(A) and (B) DIC and scanning electron microscopy images of cotyledon epidermis; arrows indicate stomatal pores. Scanning electron micrographs taken at ×480 magnification.

(C) PRO_KAT1:GUS in cotyledon epidermis.

(D) E1728:GFP in cotyledon epidermis.

(E) and (F) DIC image induced of Col and PRO_EST:FAMA root epidermis, respectively.

(G) and (H) Col and PRO_EST:FAMA leaf mesophyll, respectively. In PRO_EST:FAMA lines, mesophyll morphology is crescent-shaped instead of spherical and the cells express E1728:GFP.

(I) Ectopic PRO_FAMA:FAMA-induced guard cells expressing KAT1:GUS. Unaffected pavement cells are visible as large crenulated cells without GUS expression.

Bars = 100 μm except in (B) (20 μm) and (E) (50 μm).