Figure 5.

Behavior of FAMA Domains in Transcriptional Activity Assay and in Planta.

(A) Diagram of deletion and DNA binding site mutation constructs tested.

(B) Protoplast-based transcriptional activation assay. Effector constructs indicated on x axis. All values were normalized to VP16-driven reporter activation values. Error bars indicate standard deviations. n = 6 independent transformations.

(C) to (G) DIC images of cotyledons of plants transformed with PRO_EST:FAMA protein variants. All images are at the same magnification.

(C) Col plants expressing PRO_EST:FAMA-EAR.

(D) Col plants expressing PRO_EST:FAMA C terminus alone. The arrow indicates paired stomata.

(E) and (F) Col plants expressing PRO_EST:FAMA_PGG at 4 and 10 d, respectively.

(G) PRO_EST:FAMA_PGG in fama-1. Bar = 50 μm.