Table 1.
Proportions of EYFP-CENH3 Immunosignals (Obtained with GFP-Cy3 Antibodies) and ∼180-bp Centromeric Repeat (pAL) FISH Signals within Leaf Nuclei Isolated from EYFP-CENH3 Transgenic A. thaliana and Sorted According to Their Endopolyploidy Level ± se
| EYFP-CENH3
|
pAL
|
|||||||
|---|---|---|---|---|---|---|---|---|
| Ploidy | Nuclei No. | Nuclei Volume (μm3) | CENH3 Signal No. | CENH3 Volume (μm3) | Nuclei No. | Nuclei Volume (μm3) | pAL Signal No. | pAL Volume (μm3) |
| 2C | 10 | 22.55 ± 2.98 | 7–10 | 1.22 ± 0.15 | 10 | 27.04 ± 2.22 | 8–12 | 3.42 ± 0.23 |
| 4C | 13 | 38.02 ± 3.41 | 6–11 | 1.11 ± 0.19 | 10 | 58.08 ± 4.11 | 7–11 | 7.25 ± 0.52 |
| 8C | 11 | 83.55 ± 8.41 | 7–13 | 1.25 ± 0.20 | 20 | 116.74 ± 10.03 | 9–14 | 9.33 ± 0.55 |
| 16C | 11 | 213.9 ± 20.5 | 9–25 | 3.19 ± 0.55 | 10 | 322.81 ± 33.2 | 10–17 | 29.22 ± 3.71 |