Figure 2.

The Function of NPS6 Is Conserved among Filamentous Ascomycetes.

(A) and (B) Deletion of NPS6 in C. miyabeanus leads to reduction in virulence to rice.

(A) Third true leaves of rice cv Nipponbare inoculated with the wild-type or Δnps6 strain (Cmnps6-2) of C. miyabeanus, 5 d after inoculation.

(B) Average length of lesions formed by wild-type and C. miyabeanus Δnps6 strains. Error bars indicate 95% confidence intervals. A statistically significant difference (P < 0.01) in size was detected (lesion size [mm]: wild type = 2.30 ± 0.21; Δnps6 = 0.73 ± 0.11).

(C) and (D) Deletion of NPS6 in A. brassicicola causes reduction in virulence to Arabidopsis ecotype Di-G.

(C) Second (top row) and third (bottom row) pairs of true leaves of Arabidopsis ecotype Di-G inoculated with the wild-type or Δnps6 (Abnps6-9) strain of A. brassicicola, 4 d after inoculation.

(D) Average vertical lengths of lesions formed by wild-type and Δnps6 strains. Error bars indicate 95% confidence intervals. A statistically significant difference in size (P < 0.01) was observed (lesion size [mm]: wild type = 3.78 ± 0.38; Δnps6 = 1.48 ± 0.25).

(E) and (F) Introduction of the NPS6 ortholog of N. crassa to the Δnps6 strain of C. heterostrophus restores wild-type virulence to maize.

(E) Third true leaves of maize cv W64A-N cytoplasm infected by Δnps6 (Chnps6-1), C. heterostrophus Δnps6 carrying N. crassa NPS6 (Chnps6-1 [NcNPS6-1]), and wild-type C. heterostrophus, 5 d after inoculation.

(F) Average vertical lengths of lesions formed by each strain. Error bars indicate 95% confidence intervals. No significant difference in size was observed between the lesions formed by wild-type and Chnps6[NcNPS6-1] strains (lesion size [mm]: wild type = 3.18 ± 0.30; C. heterostrophus Δnps6 = 1.35 ± 0.18; Chnps6[NcNPS6-1] = 3.23 ± 0.25).