Figure 1.
Identification of the NDR1 and RIN4 Interaction by a Yeast Two-Hybrid Screen.
A specific interaction between NDR1 and RIN4 was identified using the CytoTrap two-hybrid system. Approximately 106 S. cerevisiae cdc25H cells comprising an Arabidopsis cDNA library made from pathogen and non-pathogen-treated tissues were screened using RIN4 (pSosRIN4) as a bait. Recapitulation of the interaction was performed on galactose-containing minimal media in the absence of uracil and leucine at 37°C (top panel). Control interactions, consisting of pSosMAFB+pMyrMAFBα (center panel, positive control) and pSOSMAFB+pMyrLamin-C (bottom panel, negative control) confirmed the NDR1–RIN4 interaction as specific.