Figure 4.

The C-Terminal RIN4 Cleavage Products Differentially Associate with RPS2 and NDR1.

Coimmunoprecipitation of RPS2:HA and HA:NDR1 with T7:RIN4_CLV-1, T7:RIN4_CLV-2, and T7:RIN4_CLV-3. Immunoblot of anti-HA and anti-T7 immunoprecipitated proteins isolated 24 h and 40 h after inoculation from wild-type N. benthamiana leaves hand-infiltrated with Agrobacterium strains expressing RPS2:HA, HA:NDR1, and RIN4-like cleavage products. Total protein extracts were immunoprecipitated with anti-HA (RPS2 and NDR1) and anti-T7 (RIN4) antibodies. Immunoprecipitated proteins were detected by immunoblotting with anti-T7 (left panels) and anti-HA (right panels) antibodies.

(A) RPS2:HA + T7:RIN4 cleavage products.

(B) HA:NDR1 + T7:RIN4 cleavage products.