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. 2006 Oct;18(10):2517–2530. doi: 10.1105/tpc.106.045484

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Copyright © 2006, American Society of Plant Biologists

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Figure 3. — Expression and Rough Fractionation of GFP-PDV1.

Immunoblot analyses using anti-GFP antibody. Homogenates of flower buds from pdv1-1 plants expressing a PDV1 transgene (lane 1) and a GFP-PDV1 transgene (lane 2) were blotted. The homogenate containing GFP-PDV1 (Total; lane 3) was centrifuged at 20,000g to sediment the low-speed pellet fraction (LSP; lane 4). The supernatant fraction was fractionated further into the high-speed pellet (HSP; lane 5) and supernatant (S; lane 6) fractions at 100,000g. The low-speed pellet fraction was treated with 0.1 M sodium carbonate (lanes 7 and 8) or 1% Nonidet P-40 (NP-40; lanes 9 and 10), and then soluble (S; lanes 8 and 10) and insoluble (P; lanes 7 and 9) fractions were separated at 100,000g.