Figure 2.

2-ME Inhibits the Release of Newly Synthesized Polypeptides from BiP.

Leaf protoplasts from wild-type tobacco were pulse-labeled with [³⁵S]Met and [³⁵S]Cys for 1 h. Chase was performed for the indicated times in the presence (+) or absence (−) of 20 mM 2-ME. BiP was immunoprecipitated with anti-BiP antiserum from protoplast homogenates and analyzed by SDS-PAGE and fluorography. The positions of BiP (arrowhead) and molecular mass markers (in kD) are indicated at left.