Figure 5.

Reintegration Sites of Osmar5NA.

(A) Scheme of plasmid rescue from ADE2 revertant genomic DNA. Yeast genomic DNA was extracted from ADE2 revertants and used to transform E. coli (see text for details). The small gray and black circles represent pOsm5NA and pOsm5Tp, respectively.

(B) Agarose gel analysis of DraI digestion of the recovered pOsm5NA derivative plasmids from (A). DNA size markers are shown at left.

(C) NdeI digestion of the plasmids used for (B).

(D) Insertion sites in pOsm5NA derivatives (pOsm5NA-d); pWL89A lacks Osmar5NA. Note that Osmar5NA has a NdeI site but not a DraI site.

(E) Insertion sites of ADE2 revertants in either the plasmid vector or yeast genomic DNA. Accession numbers of yeast genomic DNA are shown at right.