Table 1.

Detection of active seprase in malignant tumor and adjacent normal tissues.

Tumor types	Tumor tissue with active seprase expression/total tumor samples, (%)	Normal tissue with active seprase expression/total normal samples, (%)	P- value
Ovarian carcinoma	15/15*(100)	0/6 (0)	<0.0001
Breast carcinoma	6/8 (75)	3/8 (37.5)	†
Colon carcinoma	3/4(75)	1/4 (25)	†
Gastric carcinoma	7/8 (87.5)	2/8 (25)	0.0117
Melanoma	12/13*(92.3)	4/9 (44.4)	0.0132
Total	43/48 (89.6)	10/35 (28.6)	<0.0001

NOTE: In tumor and adjacent normal tissue samples seprase was isolated using WGA column and identified by parallel gelatin zymography and Western immunoblotting, as shown in Fig. 5C-F.

^*indicates that six of 15 ovarian carcinoma samples and four of 13 melanoma samples were analyzed by isolating active seprase via immunoprecipitation and subjected to soluble enzymatic assay for detection of activity, as represented in Fig. 4E-F. Significance of active seprase expression in tumor versus normal samples, indicated by P-value, was determined by Chi-square test.

^†indicates that an accurate P value could not be calculated due to small sample size; however these values contribute to the overall significance calculation.